Figure 6.

Treatment with antioxidants reduces lateral root number and DCF signal in tt4-2.A, Lateral root number was evaluated after a 3-day treatment with 1 mM N-acetyl-cysteine. Data is summarized from three independent experiments with an n of: Col-0 (control: n = 50; NAC: n = 50), tt4-2 (control: n = 49; NAC: n = 45), tt4-11 (control: n = 45; NAC: n = 45), fls1-3 (control: n = 30; NAC: n = 45), tt7-2 (control: n = 45; NAC: n = 50). B, Lateral root number was evaluated after a 3-day treatment with 500 μM ascorbic acid. Data summarized from four independent experiments with varying total numbers: Col-0 (control: n = 93; ascorbic acid: n = 89), tt4-11(CHS-GFP) (control: n = 43; ascorbic acid: n = 36), tt4-2 (control: n = 74; ascorbic acid: n = 39), tt4-11 (control: n = 48; ascorbic acid: n = 55), fls1-2 (control: n = 44; ascorbic acid: n = 40) and tt7-2 (control: n = 44; ascorbic acid: n = 38). C, Eight-day-old seedlings were stained with DCF and immediately imaged at the same laser power, gain, and pinhole for three separate experiments. DCF is oxidized by most ROS, so is a general ROS sensor. The DCF signal is reported directly as an overlay to the DIC and is also shown as a heatmap using the Zen LUT feature. Lateral root primordia were stage 4 and older due to limited stain uptake in primordia younger than stage 4. Scale bar: 50 μm. D, DCF quantification data is summarized with a total n: Col-0 (control: n = 13; ascorbic acid: n = 19), tt4-2 (control: n = 15; ascorbic acid n = 21), tt7-2 (control: n = 18; ascorbic acid: n = 17). Statistics were measured using a two-way ANOVA with a post-hoc Tukey test. Bars with the same letter represent no statistical difference, while different letters indicate statistical significance with a p < 0.05.