Table 1.
The primer sequences used for RT-qPCR
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| β-Actin | CATGTACGTAGCCATCCAGGC | CTCTTTGATGTCACGCACGAT |
| PKR | AAGCAGGAGGCAAGAAACG | TGACAATCCACCTTGTTTTCGT |
| RIG-I | ATTCAGGAAGAGCCAGAGTGTC | GTCTTCAATGATGTGCTGCAC |
| MDA5 | CGATCCGAATGATTGATGCA | AGTTGGTCATTGCAACTGCT |
| IFNβ | CCCTATGGAGATGACGGAGA | ACCCAGTGCTGGAGAAATTG |
| ISG15 | AGGTCTTTCTGACGCAGACTG | GGGGCTTTAGGCCATACTCC |
| B2 RNA | GAGTTCAAATCCCAGCAACCA | TACACTGTAGCTGTCTTCAGACA |
| IFNAR1 | GACAACTACACCCTAAAGTGGAG | GCTCTGACACGAAACTGTGTTTT |
| IFNAR2 | TGTCTGCGAGCCTAGAGACTA | AGCCGGGAATTTCGTATTGTTAT |
| JAK1 | ACGCTCCGAACCGAATCATC | GTGCCAGTTGGTAAAGTAGAACC |
| TyK2 | TGCATCCACATCGCACACAA | CTCCTGGGGATTCATGCCA |
| STAT1 | GCTGCCTATGATGTCTCGTTT | TGCTTTTCCGTATGTTGTGCT |
| STAT2 | CTGAAGGACGAACAGGATGTC | CAGGGTGGTTAATCGGCCAA |
| p16 | CGCAGGTTCTTGGTCACTGT | TGTTCACGAAAGCCAGAGCG |
| p19 | ATGCTGGATTGCAGAGCAGTA | ACGGGGCACATTATTTTTAGTCT |
| p21 | CGAGAACGGTGGAACTTTGAC | CAGGGCTCAGGTAGACCTTG |
| Cyclin E1 | CCTCCAAAGTTGCACCAGTTTGC | GACACACTTCTCTATGTCGCACC |
| Cyclin A2 | CAGTCACAGGACAGAGCTGG | GGGCATGTTGTGGCGCTTTG |
| Cyclin B1 | CGAGAACTGCTCTTGGAGACATTG | CCTGACACAGATACTCTTCTGCAG |