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. 2021 Jan 7;296:100259. doi: 10.1016/j.jbc.2021.100259

Figure 1.

Figure 1

Liver-specific (LS) hepcidin knockout (LS-Hepc−/−) mice.A–C, representative light micrographs of retinas acquired from wild-type mice (C57BL/6J) (A) and LS-Hepc−/− mice (B and C) age 12 months (female). Peripapillary retina (A and B) and posterior retina (C). D, short-wavelength fundus autofluorescence (SW-AF; 488 nm excitation) and near-infrared (NIR-AF; 790 nm) fundus autofluorescence images of wild-type (WT) and LS-Hepc−/− mice (KO). Age 12 months. Note the parapapillary hyperautofluorescent foci with 488 nm fluorescence. E, quantitation of SW-AF (quantitative fundus autofluorescence, qAF) and NIR-AF at age of 6 and 12 months. Values are means ± SD, four mice per group. F, HPLC quantitation of bisretinoid in liver-specific hepcidin knockout (LS-Hepc−/−) and wild-type (LS-Hepc+/+) mice (male gender). UV–visible chromatographic detection of the bisretinoids A2E, isoA2E, A2-dihydropyridine-phosphatidylethanolamine (A2-DHP-PE), all-trans-retinal dimer phosphatidylethanolamine (atRAL-di-PE), and A2-glycerophosphoethanolamine (A2-GPE) in LS-Hepc+/+ and LS-Hepc−/− mice. Insets at right: UV–visible spectra of chromatographic peaks corresponding to the indicated bisretinoids. G, Picomoles of A2E per eye are plotted, age 12 months. Values are based on four (wild-type) and three (LS-Hepc−/−) samples (male gender), three eyes per sample. p value was determined by unpaired two-tailed t-test.