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. 2021 Jan 7;296:100259. doi: 10.1016/j.jbc.2021.100259

Figure 4.

Figure 4

Iron, ferritin light chain (ferritin-L), and transferrin receptor (Tfrc) quantitation in neural retina (NR) and retinal pigment epithelium (RPE) of Abca4−/−and wild-type mice.A, iron quantitation by ferrozine-based colorimetric assay. Values are means ± SD; two eyes per sample; n = 3. p values were determined by one-way ANOVA and Tukey’s multiple comparison test. B, transferrin receptor (Tfrc) mRNA expression in mouse neural retina and RPE/choroid. Albino Abca4−/− mice, age 8 months. Tfrc was significantly downregulated in the neurosensory retina. Values are means ± SD of one eye per mouse; n = 4. p-values were determined by two-tailed t-test. WT versus Abca4−/−: NSR (neurosensory retina), ∗p < 0.01; RPE p > 0.05. C, Ferritin-L expression detected by immunohistochemistry in retinal cryosections. Ferritin-L (FTL) labeling is present in the neurosensory retina and RPE of both wild-type (WT) and Abca4−/− mice age 8 months. D, quantification of the mean pixel intensity in the RPE and photoreceptor layers indicates significantly increased ferritin-L positivity in the Abca4−/− mice. Values are means ± SD of one cryosection per mouse; n = 7. WT versus Abca4−/−: ∗p < 0.001, two-tailed t-test. Groups were composed of female and male mice and were gender-matched.