Table 2.

Half time of reaction (t_1/2) for PES modification of Cys-574 and Cys-603 under different conditions

Protein state	t1/2 (h)
hHsp70-ADP	4.82 ± 0.21
hHsp70-Peptide	ND
hHsp70 SBD(385–641)	2.45 ± 0.10
hHsp70 SBD(385–641)-Peptide	ND
hHsp70 NBD(1–385)-ADP	ND
hHsp70 T204A-ADP	5.23 ± 0.31
hHsp70 T204A-ATP	ND
hHsc70-ADP	19.30 ± 1.96
hHsp70-hHsc70(a)-ADP	8.56 ± 0.30
hHsc70 SBD(386–646)	4.99 ± 0.19
hHsp70 SBDα(511–641)	ND
hHsp70 SBDα(537–610)	4.71 ± 0.10

hHsc70, human HspA8; hHsp70, human HspA1A.