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Electrophoresis by 4% to 15% SDS‐PAGE. Experimental conditions: 90 minutes incubation at 37°C, pH 7.1; Lane 1: Control test tubes run with 50 μg decorin; Lane 2: 50 μg decorin combined with 0.8 μg Clostridium histolyticum collagenase; Lane 3: 50 μg decorin combined with 0.8 μg Vibrio alginolyticus collagenase; and Lanes 4 and 5: Control test tubes run with collagenase preparations only (0.8 μg)
