Table 3.

Prestin mRNA expression level on days 3 and 7 after irradiation (2^-ΔΔCT); two-way ANOVA with Sidak’s multiple comparisons test

Group	Ear	Day		P c
		3	7
Control (C)	6	0.054 ± 0.016	0.054 ± 0.001	2.032
Melatonin (M)	12	0.056 ± 0.009b	0.043 ± 0.003b	0.071
5 mg/kg M + R	12	1.006 ± 0.003a,b	1.001 ± 0.001a,b	0.920
50 mg/kg M + R	12	0.853 ± 0.014a,b	0.753 ± 0.014a,b	0.001
Radiotherapy (R)	12	1.742 ± 0.089a	2.714 ± 0.013a	0.002

^a P < 0.05 when compared to the control group at the same time point.

^b P < 0.05 when compared to the radiotherapy group at the same time point.

^cIndependent samples t-test: ^*P < 0.05 when with the same group at a different time point.