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. 2021 Jan 7;296:100237. doi: 10.1074/jbc.RA120.016069

Figure 1.

Figure 1

Methylated PP2A/Bα enzymes coimmunoprecipitate with Fyn.A, coimmunoprecipitation of endogenous PP2Ac and Fyn from total mouse cortical lysates. B, HA immunoprecipitates and total lysates from N2a cells stably expressing HA-tagged PP2Ac (WT), the methylation-incompetent L309Δ PP2Ac mutant (L309Δ), or empty vector (EV) were immunoblotted for Fyn, HA, or PP2Ac. C, flag immunoprecipitates and total lysates from N2a cells transfected with the indicated plasmids were immunoblotted for Fyn, PP2Ac, and PP2A-Bα. D, the whole NP-40 detergent-insoluble fraction prepared from GFP–Fyn–expressing N2a cells was divided into two equal parts. GFP or PP2Ac immunoprecipitates were prepared from each aliquot and analyzed for the presence of Fyn and PP2Ac (top panel). No Fyn or PP2Ac was found in control GFP immunoprecipitates carried out in EV-transfected, compared with, GFP–Fyn–expressing N2a cells (bottom panel). Representative blots from 3 separate experiments are shown in panels A–D. HA, hemagglutinin; IgG, immunoglobulin; PP2A, PP2A, phosphatase 2A; PP2Ac, catalytic “C” subunit of PP2A; N2a, Neuro-2a.