Figure 3.

Altering PP2A methylation induces defects in Fyn localization and F-actin organization in N2a cells.A, representative confocal images of the distribution of GFP–Fyn and F-actin in EV (control), WT-, L309Δ-, LCMT1-, or PME1-expressing N2a cells cotransfected with GFP–Fyn. B, Pearson’s correlation coefficients (mean ± SD, n = 12 cells/transfection from three separate experiments) showing colocalization of Fyn and F-actin in these cells. Data were analyzed using one-way ANOVA (F (4, 55) = 18.62, p < 0.0001) with post hoc Dunnett’s test; ∗∗∗p < 0.001, versus EV. C, cells were also analyzed for the length of actin-positive protrusions. Data (mean ± SD) were appraised using one-way ANOVA (F (4, 928) = 785; p < 0.0001) with post hoc Dunnett’s test. ∗∗∗∗p < 0.0001, versus control. D, F-actin distribution in the indicated N2a cell lines in the absence of GFP–Fyn. Images in panels A and D are representative of three separate experiments. Scale bars, 5 μm. EV, empty vector; N2a, Neuro-2a; LCMT1, leucine carboxyl methyltransferase 1; PP2A, protein phosphatase 2A.