Figure 3.

Voltage-dependent rectification and pharmacology of Piezo1 pore domain.A, the current–voltage relationship of full-length Piezo1 (black), Piezo1 deletion proteins starting from S1953 (yellow), G2124 (red), and E2172 (green), negative control no-transfection (gray) elicited by negative pressure (−15 mmHg). The inset demonstrates the ionic composition of pipette and bath solutions used for all the experiments in an inside-out patch configuration. B, comparison of single-channel conductance between full-length Piezo1 and indicated pores at ±60 mV showing voltage-dependent rectification (n ≥ 9). The data and statistics represented as Mean ± Standard Deviation, One-way ANOVA, and two-tailed t-test where ∗∗p < 0.005. C, representative multiple-channel currents of full-length Piezo1 elicited by negative suction in the absence (upper trace, n = 12 cells) and presence (lower trace, n = 12 cells) of 4 μM extracellular GsMTx-4 toxin, acquired at −60 mV. D, representative multiple-channel currents of E2172 pore domain elicited by negative suction in the absence (upper trace, n = 8 cells) and presence (lower trace, n = 8 cells) of 4 μM extracellular GsMTx-4 toxin, acquired at −60 mV.