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. 2020 Dec 29;296:100225. doi: 10.1074/jbc.RA120.015905

Figure 5.

Figure 5

The pressure-dependent open probability of full-length Piezo1 and pore domain.A, left to right, full-length Piezo1 EM structure. Single-channel currents at ± 60 mV at 0 and −30 mm Hg in cell-attached mode, arrow indicates application of negative pressure, O and C denote open and closed state of the channel. All-point current histograms at ± 60 mV with and without pressure application. Normalized open probability bar graphs at +60 mV (top) and −60 mV (bottom). The open probability is presented as Mean ± Standard Deviation, two-tailed t-test: ∗∗∗p < 0.0005. B, left to right, Piezo1 pore domain E2172 representation based on full-length Piezo1 structure. Single-channel currents at ±60 mV acquired at 0 and −30 mm Hg in cell-attached mode, arrow indicates application of negative pressure, O and C denote open and closed state of the channel. All-point current histograms at ±60 mV with and without pressure. Normalized open probability bar graphs at +60 mV (top) and −60 mV (bottom). The open probability is presented as Mean ± Standard Deviation, one-tailed t-test: ns p > 0.06 at +60 mV, p > 0.98 at −60 mV.