Figure 1.
C12 and DDDA diets impair long-term hepatoblastoma (HB) growth and induce massive necrosis.A, survival curves of HB-bearing mice. Each of the indicated cohorts was injected with Sleeping Beauty vectors encoding Δ(90) and YAPS127A (19, 20, 21, 22, 27). One week later, the indicated diets were initiated for the remainder of the study. Kaplan–Meier survivals for each group were determined by the log-rank test. B, tumors were generated as described in A except that mice were maintained on standard diets for 8 weeks. Half of the mice were then provided DDDA diets, and the remaining mice were maintained on standard diets. MRIs were performed on each group at week 11. Representative axial sections from four mice in each group are shown. C, low-power magnification (5×) of a typical H&E-stained section from a standard diet HB obtained at the time of sacrifice. D, similar H&E section of a typical HB from a mouse maintained for 3 weeks on a DDDA diet as described in B. A large area of necrosis extending from the tumor surface into the parenchyma is indicated by the arrow. DDDA, dodecanedioic acid.