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. 2021 Jan 13;296:100283. doi: 10.1016/j.jbc.2021.100283

Figure 5.

Figure 5

Oxygen consumption rates (OCRs) in livers and tumors from mice maintained on standard, C12-, or DDDA-enriched diets. Mitochondria were isolated from livers and hepatoblastomas of mice maintained on the indicated diets (Fig. 2A), and OCRs were determined as previously described (18, 19, 20, 21, 22). A, total oxidative phosphorylation (Oxphos) in response to the addition of cytochrome c, malate, ADP, pyruvate, glutamate, and succinate. B and C, complex I and complex II activities activity. D, the glutamate response was determined after the addition of cytochrome c, malate, ADP, and pyruvate. E, OCRs in response to palmitoyl-CoA were determined following the addition of cytochrome c, malate, ADP, and l-carnitine. F, OCRs in response to l-carnitine were determined following the addition of cytochrome c, malate, and ADP. G, the fraction of β-FAO that was Cpt1a dependent was determined following the addition of etomoxir to palmitoyl-CoA– and l-carnitine–supplemented reactions as described in E. DDDA, dodecanedioic acid.