Figure 7.

1,25D and FoxO1 deletion ameliorates osteoblasts autophagy in high-glucose environment.A, variations of protein levels of FoxO1, Beclin1 and LC3II/LC3I assessed by western blot in different groups, n = 5. B, representative images and quantification analysis of mRFP-GFP-LC3 fluorescence double labeling, scale bar=5 μm, each image is representative of four specimens. Red indicates the number of autolysosomes in osteoblasts exposed to different treatment, and yellow refers to the number of autophagosomes in cells. Data are expressed as mean ± standard deviation (SD) obtained in 3 ihreendependent experiments, ns represents p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. FoxO1_KO, conditional knockout of FoxO1; FoxO1_Len, lentivirus-mediated overexpression of FoxO1; HG, high glucose.