Figure 2.

Fluorescence anisotropy of ferric uptake regulator (Fur) and PhuS binding.A, Mn–Fur binding to the 5′-FAM–labeled prrF1-30. B, apo-PhuS binding to the 5′-FAM–labeled prrF1-50 and prrF1-30 color coded as shown. Experiments were performed in triplicate as described in Experimental procedures section. The data were fit by converting the anisotropy, r, to fraction bound and plotted against protein concentration using a one-site binding model. The error is shown as the SEM. 5′-FAM, 5′-fluoroscein amidite.