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EMSA of apo- and holo-PhuS binding to prrF1-50. A, apo-PhuS binding to 5′-biotin–labeled prrF1-50. B, holo-PhuS binding to 5′-biotin–labeled prrF1-50. Experiments were performed as described in Experimental procedures section. All reactions contained a fixed concentration (30 pM) of labeled prrF1-50, and the following incubation was run on 8% acrylamide gels and transferred to a nylon membrane and visualized by chemiluminescence.
