Figure 5.

Fluorescence anisotropy (FA) and EMSA of apo-PhuS H212R binding to prrF1-50.A, FA of apo-PhuS H212R binding to the 5′-FAM–labeled prrF1-50 as described for Figure 2. The data were fit by converting the anisotropy, r, to fraction bound and plotted against protein concentration using a one-site binding model. The error is shown as the SEM. B, apo-PhuS H212R binding to 5′-biotin–labeled prrF1-50 as described for Figure 3. All reactions contained a fixed concentration (30 pM) of labeled prrF1-50, and the following incubation was run on 8% acrylamide gels, transferred to a nylon membrane, and visualized by chemiluminescence.