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. 2021 Jan 9;296:100275. doi: 10.1016/j.jbc.2021.100275

Figure 6.

Figure 6

Relative PrrF1 and PrrH sRNA levels for PAO1 WT, ΔphuS, and the phuSH212R allelic strain in iron-deplete or heme-supplemented conditions.A, PrrF1 relative sRNA levels. B, PrrH relative sRNA levels. mRNA isolated at 0, 2, and 5 h following growth in either iron-deplete M9 or M9 supplemented with 1 μM heme. mRNA values represent the mean from three biological experiments, each performed in triplicate and normalized to 0 h. Gray shaded bars represent iron-deplete conditions; red shaded bars represent heme-supplemented conditions. Error bars represent the standard deviation from three independent experiments performed in triplicate. p values as determined by two-tailed Student's t test comparing values upon heme supplementation to iron-deplete conditions at the same time point, where ∗p < 0.05.