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. 2020 Nov 22;296:100030. doi: 10.1074/jbc.RA120.015911

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Interactions between HO-1 and different P450 enzymes. Screen of various cytochromes P450 for their potential interaction with heme oxygenase-1. HEK 293T/17 cells were transfected with vectors coding for Rluc-tagged HO-1 and GFP-tagged P450. Expression of GFP-tagged protein was between 15- and 30-fold higher than HO-1-Rluc expression for each pair in order to approach a maximal BRET response. Error bars represent the standard deviation (SD) for triplicate measurements of cells from a single transfection. BRET, bioluminescence resonance energy transfer; GFP, green fluorescent protein; HO-1, heme oxygenase 1.