Table 2.
Enzyme kinetics raw data for ASFV dUTPase and swine dUTPase
| Enzyme | Km (μM) | Kcat (s−1) | kcat/Km (M−1 s−1) | Relative efficiency (%) |
|---|---|---|---|---|
| aDUTWT | 0.98 ± 0.2 | 4.37 ± 0.15 | 4.46 × 106 | 100% |
| aDUTD29A | 6.89 ± 0.2 | 0.31 ± 0.1 | 4.62 × 104 | 1.04% |
| aDUTR71A | ∖ | ∖ | ∖ | ∖ |
| aDUTS72A | 4.89 ± 0.35 | 0.48 ± 0.2 | 9.85 × 104 | 2.20% |
| aDUTS73A | 4.16 ± 0.19 | 5.58 ± 0.3 | 1.34 × 106 | 29.99% |
| aDUTL89A | 5.44 ± 0.5 | 2.94 ± 0.7 | 0.54 × 106 | 34.17% |
| aDUTI90A | 0.47 ± 0.26 | 0.75 ± 0.2 | 1.58 × 106 | 35.37% |
| aDUTD91A | ∖ | ∖ | ∖ | ∖ |
| aDUTY94A | ∖ | ∖ | ∖ | ∖ |
| aDUTQ120A | 16.76 ± 1.21 | 0.58 ±0.4 | 3.51 × 104 | 0.79% |
| aDUT△147 | ∖ | ∖ | ∖ | ∖ |
| aDUTR149A | ∖ | ∖ | ∖ | ∖ |
| aDUTR149K | 4.51 ± 0.7 | 0.15 ± 0.1 | 0.33 × 105 | 0.73% |
| aDUTF154Y | 26.45 ± 0.2 | 1.5 ± 0.1 | 0.56 × 105 | 1.26% |
| sDUTWT | 0.58 ± 0.13 | 5.58 ± 0.6 | 9.60 × 106 | 100% |
‘∖’: no activity or diminished the enzymatic activity to an undetectable level.