Table 2.
Observed LC-MS-MS peaks of trypsin-digested recombinant PE peptides
| Samplea | α-82 | α-139 | β-82b | β-165 | β-50, 61b |
|---|---|---|---|---|---|
| A/B+S+PEB | NDc | Unmodd | PEB (91.1%) | Unmod | Unmod |
| A/B+V+PEB | Unmod | Unmod | Unmod | ND | Unmod |
| A/B+V+S+PEB | ND | Unmod | PEB (95.3%) | Unmod | PUB (31.3%) |
| A/B+ZS+PEB | ND | Unmod | PEB (92.1%) | Unmod | Unmod |
| A/B+V+ZS+PEB | ND | Unmod | PEB (98.2%) | Unmod | PUB (29.4%) |
| Sample | α-75 | α-83 | α-140 | β-82 | β-159 | β-50, 61 |
|---|---|---|---|---|---|---|
| MA/B+V+S+PEB | ND | ND | ND | PEB | ND | Unmod |
| MA/B+V+ZS+PEB | ND | ND | ND | PEB (99.7%) | ND | PUB (84.7%) |
a
A/B, pHTCpeA/HTCpeB; S, pCpeS; V, pHTMpeV; ZS, pHTCpeZ/CpeS; MA/B, pHTMpeA/HTMpeB.
b
Parenthetical values represent calculated percentage of observed peptides modified by bilin as indicated. These results are representative of two independent biological replicates.
c
ND represents peptides that were not detected.
d
Unmod represents peptides that were unmodified and lack a bilin chromophore.