Figure 4.

HRI controls responses to aggregating α-synuclein.A, HEK293T cells transduced with lentiviral particles targeting either a scrambled sequence (SC) or HRI (shHRI) were transfected with α-synuclein fused to a truncated luciferase (αsyn L1) and either pcDNA or α-synuclein fused to the remaining luciferase fragment (αsyn L2) and luciferase assays were performed on the cells (left) and the conditioned media (right). Bar graphs display means ± SEM plotted from six independent experiments pooled from two biological sets of three each (N= 6). B, HEK293T cells transduced with lentiviral particles targeting either a scrambled sequence (SC) or HRI (shHRI) were transfected with αsyn L1 and either pcDNA or αsyn L2 and analyzed by Western blotting using the indicated antibodies. Coimmunoprecipitation assay using lysates of HEK293T cells transduced with lentiviral particles targeting a scrambled (SC) sequence or HRI (shHRI), transfected with GFP tagged α-synuclein following stimulation for 4 h with 5 μM MG132. ∗ and ∗∗∗ indicates p < 0.05 and 0.001, respectively.