Figure 6.

Accumulation of α-synuclein in the central nervous system of aged Hri^−/−mice.A, whole brain lysates from 7-month-old Hri^−/− and Hri^+/+ littermate mice (N = 3 mice per group) analyzed by Western blotting using the indicated antibodies. B–C, representative images of wildtype and HRI knockout mice spinal cord coronal sections stained with anti P-S129 α-synuclein antibody (B) and BAG3 (C) (N = 6 mice per group). In C, the black bold arrows indicate BAG3+ puncta in proximity of neuronal nuclei, whereas the black dashed arrows indicate neurons that did not show BAG3+ puncta. In C, dot plot represents means ± SEM of perinuclear BAG3+ puncta/mm² of spinal cord gray matter from Hri^−/− and Hri^+/+ littermates (N = 6 mice in each group). The scale bar represents 50 μm.