Table 1.
Target, host and source of primary and secondary antibodies employed for immunofluorescent islet histology and immunocytochemistry studies.
| Primary antibodies | ||||
|---|---|---|---|---|
| Target | Host | Dilution | Source | |
| Insulin | Mouse | 1:500 | Abcam, ab6995 | |
| Glucagon | Guinea pig | 1:200 | Raised in-house, PCA2/4 | |
| GFP | Goat | 1:400 | Abcam, ab5450 | |
| Secondary antibodies | ||||
| Target | Host | Reactivity | Dilution | Fluorescent dye and source |
| IgG | Goat | Mouse | 1:400 | Alexa Fluor® 594, Invitrogen, UK |
| IgG | Goat | Guinea pig | 1:400 | Alexa Fluor® 594, Invitrogen, UK |
| IgG | Goat | Guinea pig | 1:400 | Alexa Fluor® 488, Invitrogen, UK |
| IgG | Donkey | Goat | 1:400 | Alexa Fluor® 488, Invitrogen, UK |