Figure 3.

Myo9b does not influence HL-60 cell differentiation to macrophages.A, Myo9b protein expression increases slightly during PMA-induced HL-60 macrophage differentiation (w/o: undifferentiated). B, flow cytometry analysis of the cell surface expression of differentiation marker CD11b in undifferentiated and differentiated HL-60 cells. No differences in the upregulation of expression were detected between wild-type and Myo9b-deficient cells. Histograms of a representative experiment are shown for a wild-type and a Myo9b-deficient cell line. C–D, quantification of CD11b-positive cells of indicated cell lines that were either undifferentiated (C) or differentiated with PMA for 1 day (D). Data are from four independent experiments, n.s., not significant by the Kruskal–Wallis test.