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. 2020 Nov 24;296:100100. doi: 10.1074/jbc.RA120.014888

Figure 3.

Figure 3

Cholesterol auxotrophic cell lines express SCARB1, and HDL NP treatment reduces cell viability and alters gene expression.A. Western blot analysis of SCARB1 expression in SUDHL1, SR-786, and U937 cells. Ramos and Jurkat represent positive and negative controls for SCARB1 expression, respectively. β-Actin was used as a loading control. B. Cell death (MTS) assay of SR-786, SUDHL1, and U937 cells treated with HDL NPs for 120 h. ∗p < 0.05 vs PBS control. C. RT-qPCR analysis of cholesterol biosynthesis genes INSIG1, DHCR7, and HMGCS1 in SR-786, SUDHL1, and U937 cells treated with HDL NPs (20 nM, 50 nM) or human HDL (50 nM) for 72 h. ∗p < 0.05 vs PBS control. D. Western blot (left) and RT-qPCR (right) analysis of GPX4 expression in SR-786, SUDHL1, and U937 cells following 72-h treatment with HDL NPs (20 nM, 50 nM) or human HDL (50 nM). ∗p < 0.05 vs PBS control. HDL NPs, high-density lipoprotein–like nanoparticles; PBS, phosphate buffered saline.