Figure 4.

HDL NPs induce ferroptosis in cholesterol auxotrophic cell lines.A–C. Flow cytometric analysis for C11-BODIPY fluorescence in SR-786 (A), SUDHL1 (B), and U937 (C) cells treated with HDL NPs (50 nM) over 72 h. ∗p < 0.05 vs 0 h. ∗∗p < 0.01 vs. 0 h. ∗∗∗p < 0.005 vs. 0 h. ∗∗∗∗p < 0.001 vs. 0 h. D–F. Cell death (MTS) assays for SR-786 (D), SUDHL1 (E), and U937 (F) cells treated with HDL NPs, ferrostatin-1 (1 μM), and/or DFO (1 μM) for 120 h. ∗p < 0.05 vs HDL NPs. DFO, deferoxamine; HDL NPs, high-density lipoprotein–like nanoparticles.