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. 2020 Nov 23;296:100085. doi: 10.1074/jbc.RA120.015824

Figure 1.

Figure 1

Purification and characterization of DDX43 KH-domain proteins.A, the schematic representation of four DDX43 KH-domain fragments. The KH domain and GRGG loop are indicated in red and helicase core domain in yellow. B, the SDS-PAGE analysis of recombinant DDX43 KH-domain proteins eluting from a Sephacryl S-100 HR column. C, the Western blot analysis for proteins shown in panel B with an anti-His antibody (SC-8036, Santa Cruz). D–E, the representative EMSA images of increasing DDX43 KH-domain protein (126 aa, 0–9.6 μM) binding with 0.5 nM of indicated DNA (D) and RNA (E) substrates. See Table S2 for substrate sequence. DNA is in black and RNA in gray. EMSA, electrophoretic mobility shift assay; HR, high-resolution.