Figure 3.

The conserved GRGG loop region is involved in nucleotide binding.A, a 600-MHz ¹H-¹⁵N HSQC spectrum of DDX43 KH-89 protein with some of the peak assignments. B, combined chemical shift changes caused by dT₅ and dT₁₀ binding to DDX43-KH domain (89 aa) as a function of amino acid. C, a model structure of DDX43 KH domain (89 aa) generated by Phyre 2. Potential amino acids involved in dT₅ binding are indicated by colors according to their combined Δδ. HSQC, heteronuclear single quantum coherence.