Figure 8.

Mutated alanine (A81) or glycine (G87) affects DDX41 in binding and unwinding processivity.A, the SDS-PAGE analysis of purified DDX43 full-length proteins (WT and mutants, 1 ug each). B–E, the representative images of EMSA performed by incubating DDX43 full-length proteins (2 μM) with 0.5 nM of 13-bp duplex RNA with 5′ tail of polyA (B), polyU (C), UUGU repeats (D), and their quantitative analysis (E). F, the quantitative analysis of helicase assays of DDX43 proteins (9.6 μM) on 0.5 nM of 13-bp duplex RNA with 5′ tail of polyA, polyU, or UUGU repeats as a function of time (0–45 min). G–J, the representative images of EMSA performed by incubating DDX43 full-length proteins (2 μM) with 0.5 nM of 20-bp duplex DNA with 3′ tail of polyA (G), polyT (H), TTGT repeats (I), and their quantitative analysis (J). K, the quantitative analysis of helicase assays of DDX43 proteins (9.6 μM) on 0.5 nM of 20-bp duplex DNA with 3′ tail of polyA, polyT, or TTGT repeats as a function of time (0–45 min). The triangle indicates heat-denatured RNA or DNA substrate control. Data are presented as the mean ± SD, n = 3. EMSA, electrophoretic mobility shift assay; NE, no enzyme.