Figure 1.

GluOC administration in adipocyte-specific GPRC6A knockout female mice does not reduce adipocyte size.A, RT-PCR analysis of GPRC6A and β-actin (internal control) mRNAs in isolated adipocytes from eWAT of control (Gprc6a^fl/fl) and adG6AKO mice. B, immunoblot analysis of GPRC6A and β-actin (loading control) in eWAT and testis (positive control) of control and adG6AKO mice. C, time course of body weight for control and adG6AKO mice during maintenance on the HFS diet for 12 weeks, beginning at 3 weeks of age. Error bars are omitted for clarity. D, time course of body weight for control and adG6AKO mice. GluOC (10 ng/g) or saline was administered daily for 6 weeks, beginning at 8 weeks of age. HFS feeding was started at the same time. E, H&E staining of eWAT from control and adG6AKO mice (left) and the determination of adipocyte area for 50 adipocytes per slide and at least three sections for each mouse (right). The bars represent 50 μm. All data are the mean ± SEM for 8 to 10 mice per group. ∗p < 0.05, ∗∗p < 0.01 for indicated comparison by two-way ANOVA (D) and Student's t-test (E). eWAT, epididymal white adipose tissue; GluOC, uncarboxylated osteocalcin, HFS, high-fat and high-sucrose.