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. 2021 Jan 9;296:100274. doi: 10.1016/j.jbc.2021.100274

Figure 3.

Figure 3

Histological analysis of adG6AKO male mice maintained on the HFS diet for 15 weeks.A, H&E staining of eWAT from control and adG6AKO mice (left) and determination of adipocyte area for 50 adipocytes per slide and at least three sections for each mouse (right). The bars represent 50 μm. B and C, serum concentrations of leptin and adiponectin, respectively, measured in the fed state. D, H&E staining of liver sections from control and adG6AKO mice (left) and determination of lipid area in at least three sections for each mouse (right). The bars represent 100 μm. E, liver triglyceride content was measured after 15 weeks of HFS feeding. F, immunostaining for insulin in pancreatic sections counterstained with hematoxylin (left) and determination of islet area in at least three sections for each mouse (right). The bars represent 100 μm. G, serum insulin levels after deprivation of food for 16 h and at 30 min after subsequent intraperitoneal injection of glucose (1 g/kg). H, blood glucose levels after deprivation of food for 5 or 16 h. All quantitative data are the mean ± SEM for 8 to 10 mice per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus the corresponding value for control mice (Student's t test). eWAT, epididymal white adipose tissue; HFS, high-fat and high-sucrose.