Figure 7.

L-serine extends CLS through the one-carbon metabolism pathway.A, schematic diagram of one-carbon metabolism is Saccharomyces cerevisiae. Enzymes that catalyze specific reactions are in bold. Transporters are boxed. B, CLS of BY4741 and shm1Δ mutant under NR and CR conditions or supplemented with 5 mM L-serine. C, CLS of BY4741 and shm2Δ mutant under NR and CR conditions or supplemented with 5 mM L-serine. D, CLS of BY4741 and mtd1Δ mutant under NR and CR conditions or supplemented with 5 mM L-serine. E, CLS of BY4741 and fsf11Δ mutant under NR and CR conditions or supplemented with 5 mM L-serine. F, mean CLS statistics from panel E calculated using OASIS 2. All CLS assays were run in triplicate.