Figure 1.

hm⁵C is enriched in tRNA. A, schematic of TET2-mediated m⁵C oxidation products. B, LC-MS/MS quantification of hm⁵C in total RNA, 18S rRNA, 28S rRNA, poly(A)-RNA, and tRNA extracted from mESCs. C, LC-MS/MS quantification of hm⁵C in total RNA, 18S rRNA, 28S rRNA, poly(A)-RNA, and tRNA extracted from mESCs HEK293T cells. p values were determined using two-tailed Student's t test for unpaired samples. Error bars represent mean ± s.d., n = 4 (four biological replicates × two technical replicates) ∗∗∗∗ p < 0.001. LC-MS/MS, liquid chromatography and tandem mass spectrometry; mESCs, mouse embryonic stem cells.