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. 2020 Nov 23;296:100087. doi: 10.1074/jbc.RA120.014226

Figure 2.

Figure 2

TET2-CS–mediated in vitro oxidation reaction. A, constructs of TET2-CS and TET2-CD. B, LC-MS/MS tracks of C, m5C, and hm5C from in vitro oxidation reaction of TET2-CS with purified tRNAs. tRNAs purified from HEK293T cells were incubated with either TET2-CS or buffer in the presence of alpha-ketoglutarate. C, LC-MS/MS quantification of C, m5C, and hm5C from in vitro oxidation reaction of TET2-CS with purified tRNAs. p values were determined using two-tailed Student's t test for unpaired samples. Error bars represent mean ± s.d., n = 3 (three biological replicates × two technical replicates). ∗ p < 0.05, ∗∗∗∗ p < 0.001. AA, atomic absorption; Cys-C, cysteine (Cys)-rich domain C terminal; Cys-N, cysteine (Cys)-rich domain N terminal; DSBH, double-stranded β-helix; LC-MS/MS, liquid chromatography and tandem mass spectrometry; N/A, not detectable; RT, retention time.