Figure 3.

TET2-mediated m⁵C oxidation inside cells. A, LC-MS/MS tracks of C, m⁵C, and hm⁵C from purified tRNAs extracted from wild-type and Tet2 KO mESCs. B, LC-MS/MS quantification of hm⁵C in tRNA extracted from wild-type and Tet2 KO mESCs. C, LC-MS/MS tracks of C, m⁵C, and hm⁵C from purified tRNAs extracted from TET2-inducible expression HEK293T cells before and after doxycycline induction. D, LC-MS/MS quantification of hm⁵C in tRNA extracted from TET2 inducible expression HEK293T cells and control HEK293T cells before and after doxycycline induction. p values were determined using two-tailed Student's t test for unpaired samples. Error bars represent mean ± s.d., n = 3 (three biological replicates × two technical replicates) ∗∗ p < 0.01, ∗∗∗∗ p < 0.001, n.s. means p > 0.05. AA, atomic absorption; KO, knock-out; LC-MS/MS, liquid chromatography and tandem mass spectrometry; mESCs, mouse embryonic stem cells; RT, retention time.