Figure 1.

BC200 knockdown inhibits translation.A, quantification of puromycin incorporation by densitometry analysis of Western blots performed with an anti-puromycin antibody following transfection of MCF-7 cells with a control or BC200 targeting LNA GapmeR. Lines represent the mean background corrected sum of lane signal intensity for each of four biological replicates. Standard deviation is represented by the dashed lines. B, relative BC200 expression was measured by RT-qPCR on 25 ng of RNA extracted from a fraction of the samples used in (A). Lines represent the mean of three biological replicates measured in duplicate. Dashed lines indicate standard deviation. C, western blot of puromycin incorporation time course used to generate the data in (A). D, polysome analysis by sucrose density gradient centrifugation of cell lysates 48 h following transfection of a control (black) or BC200 targeting LNA GapmeR (red). Profiles are representative of at least three independent replicates.