Figure 4.

Transient plasmid transfection impairs mRNA translation.A, polysome analysis by sucrose density gradient centrifugation of cell lysates 96 h following transfection of a GFP reporter plasmid (blue) as compared with untreated cells (black). B, as in (A), comparison of a BC200 expression plasmid (red) with untreated cells (black). C, overlay of polysome profiles from cells transfected with a GFP reporter plasmid (blue) with the same plasmid containing a BC200 expression cassette (red). D, quantification of BC200 expression by RT-qPCR analysis of DNase I-treated RNA extracted from the samples used in A–C. Horizontal line represents the mean of three replicates ± standard deviation.