Figure 5.

Stable transfection of BC200 elevates the fraction of polysomal RNA.A, polysome analysis by sucrose density gradient centrifugation of cell lysates 48 h following plating 15 × 10⁶ cells into 150-mm dishes. Polysome profile from wild-type MCF-7 cells (black) is overlaid with a profile from cells stably selected to express a GFP reporter plasmid (red). B, as in (A), overlaid polysome profiles of cells stably expressing a GFP reporter plasmid (black) with cells stably expressing a GFP reporter plasmid with a BC200 expression cassette (red). C, as in (B) with cells stably expressing a GFP reporter plasmid with a BCMUT expression cassette (red). D, as in (B) with cells stably expressing a GFP reporter plasmid with a G22 expression cassette (red).