Figure 1.

Drosophila pod1 genetically interacts with Hippo pathway genes.A, schematic diagram of the genetic screen for Hippo pathway regulators. B, representative pictures of the wings of Pod1 overexpression (Pod1), pod1 knockdown (pod1^RNAi), pod1 knockout (pod1^KO) without or with Hpo knockdown (+Hpo^RNAi) or Yki:GFP (+Yki) overexpression by MS1096-GAL4. The PCV is indicated. The scale bar represents 500 μm. C, statistical analysis of the wing size of each genotype in (B). The error bars represent ± S.D. from n > 20. One-way ANOVA and Tukey post-test were applied (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001). D, posterior regions of eye discs in Drosophila larvae. Yki:GFP, Yki^S168A:GFP, Scalloped, and pod1^RNAi were expressed by GMR-GAL4 as indicated. The scale bar represents 10 μm. GMR, glass multimer reporter; PCV, posterior cross vein.