Figure 6.

Tyrosine kinase Src is a potential regulator of CORO7.A, representative pictures of the wings expressing RNAi targeting Src64B (Src64B^RNAi) with or without expressing Pod1 or RNAi targeting pod1 (pod1^RNAi) by MS1096-GAL4 as indicated. The scale bar represents 500 μm. B, statistical analysis of the wing size of each genotype in (A). The error bars represent ±S.D. from n > 20. One-way ANOVA and Tukey post-test were applied (∗p < 0.05; ∗∗∗p < 0.001). C, siRNA targeting CORO7 (si-CORO7) or nontargeting control siRNA (si-control) was transfected in MDA-MB-231 cells, and 50 nM dasatinib was treated for 1 h as indicated. The cell lysate samples were immunoblotted with anti-pYAP, anti-YAP, antitubulin, and anti-CORO7 antibodies. Quantification of the fold ratio of pS127-YAP bands to YAP protein levels under each indicated condition (right). The error bars represent ±S.D. from n = 3. One-way ANOVA and Tukey post-test were applied (∗∗p < 0.01). D, HEK293T cells expressing Flag-CORO7 and HA-Src were treated with 250 nM dasatinib for 2 h. The cell lysate samples were immunoprecipitated with anti-Flag antibody and immunoblotted with anti-pTyr and anti-Flag antibodies. The WCL samples were loaded for indicating the expression levels. E, HEK293T cells were transfected with indicated mutant forms of Flag-CORO7 and a constitutively active form of Src (HA-Src Y530F). CORO7 3YF indicates that Tyr712, Tyr738, and Tyr758 were all mutated to phenylalanine. The lysate samples were immunoprecipitated with anti-Flag antibody and immunoblotted with anti-pTyr and anti-Flag antibodies. The WCL samples were loaded for indicating the expression levels. CORO7, coronin 7; YAP, yes-associated protein; HA, hemagglutinin; WCL, whole cell lysate.