Figure 2.

EC-specific SOCS3 knockout exacerbates HKSA or IL-6-induced ALI. A, wild-type (WT) or EC-specific SOCS3 knockout (SOCS3 KO) mice were injected with HKSA (intratracheally, 2 × 10⁸ bacterial cells/mouse), and after 18 h vascular leak was determined by Evans blue extravasation assay. Normal saline was used as a vehicle. Bar: 4 mm. B, the total cell count (left) and total protein content (right) were determined in bronchoalveolar lavage (BAL) fluid. Bar diagrams present individual data points, means (cross), and medians (line); n = 8, ∗p < 0.05. C, WT and SOCS3 KO mice were injected with IL-6 (i.v., 100 ng/mouse) and Evans blue extravasation assay was performed. Bar: 4 mm. D, cell count and protein content were measured in BAL; n = 6, ∗p < 0.05. The bar diagrams represent individual data points and averages with standard error.