Figure 3.

SOCS3 overexpression attenuates HKSA or IL-6-induced endothelial barrier disruption and inflammation. A and B, ECs were transfected with Myc-tagged SOCS3 or left nontransfected (Non-TF) followed by HKSA (A) or IL-6 (B) treatment. TER was monitored over 20 h. C, western blot analysis of phospho-NFκB, VCAM-1, and ICAM-1 levels in HKSA-treated EC with ectopic expression of Myc-SOCS3 and in nontransfected controls. Probing with β-actin was used as a loading control. Reprobing with Myc antibody was used to verify ectopic SOCS3 overexpression. Bar graphs depict analysis of western blotting data by quantitative densitometry; n = 5, ∗p < 0.05. RDU, relative density units.