Figure 2.

The human αS A30P mutant is predominantly cytosolic. Cytosolic and membrane fractions were sequentially extracted from cortical neurons expressing human wt or A30P αS at 37 °C (A). The solubility of control proteins GAPDH and calnexin is depicted in panel B. Representative western blot images to the quantification are shown in panel C. Digitonin concentration, 800 or 900 μg/ml. (C, cytosol and M, membrane). N = 3 independent experiments, performed on different days in n = 12 + 4 + 4 independent wells (total n = 20). ∗∗∗∗p < 0.0001. ns, not significant.