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. 2020 Nov 23;296:100034. doi: 10.1074/jbc.RA120.014126

Figure 1.

Figure 1

ST6Gal-I is upregulated in S2-LM7AA and S2-013 metastatic subclones, which display enrichment in gene networks associated with stemness and EMT. A, ST6Gal-I is expressed in most human PDAC cell lines, with the exception of Suit2 cells. B, ST6Gal-I is upregulated in Suit2-derived metastatic subclones, S2-013 and S2-LM7AA. C, ST6Gal-I was overexpressed (OE) in parental Suit2 cells. Control lines were transduced with an empty vector (EV). Both lines represent stable, polyclonal populations. Densitometric values in A–C were normalized to their respective loading controls. D–E, RNA-Seq data collected from S2-LM7AA (D) and S2-013 (E) lines were compared with data from Suit2 EV cells. The top 20 Biological Functions altered in S2-LM7AA and S2-013 cells are shown. Red bars denote Functions that are shared between the two lines. F–G, GSEA of S2–LM7AA (F) and S2-013 (G) cells relative to Suit2 EV cells revealed enrichment in the Wnt, Hedgehog, EMT, and Hypoxia pathways. The normalized enrichment score (NES) and false discovery rate (FDR) values for S2-LM7AA cells are: Wnt, NES = 1.47; FDR = 0.05; Hedgehog, NES = 1.64; FDR = 0.015; EMT, NES = 2.22; FDR < 0.0005; and Hypoxia, NES = 1.76, FDR = 0.005. For S2-013, the values are: Wnt, NES = 1.18; FDR = 0.33; Hedgehog, NES = 1.95; FDR < 0.0005; EMT, NES = 1.65; FDR = 0.012; and Hypoxia, NES = 2.00, FDR < 0.0005.