Figure 5.

Cells with high ST6Gal-I expression exhibit increased α2-6 sialylation and activation of EGFR. Suit2 (A), S2-LM7AA (B), and S2-013 (C) cells with modulated ST6Gal-I expression were evaluated for sialylation and activation of EGFR. ST6Gal-I-sialylated proteins were precipitated using SNA lectin and then immunoblotted for EGFR. To evaluate EGFR activation state, whole-cell lysates were immunoblotted for phosphorylated (pY1068) or total EGFR. Densitometric values were normalized to the β-tubulin loading control.