Figure 6.

EGFR inhibition attenuates the invasive phenotype conferred by ST6Gal-I in Suit2 and S2-LM7AA cells. A, Suit2, B, S2-LM7AA, and C, S2-013 cells were treated for 24 h with erlotinib (+erl) or left untreated (UT). Cell lysates were immunoblotted for phospho and total EGFR. Short and long film exposures are shown for the phospho-EGFR blots. Densitometric values in A–C were normalized to the loading control. D, Suit2 parental and metastatic cell lines were treated with or without erlotinib and then subjected to invasion assays. Representative images are shown of crystal violet–stained cells. E, crystal violet–stained cells were solubilized with 10% acetic acid, and relative invasion was quantified by absorbance spectroscopy. Absorbance values were normalized to those of controls (cells seeded in chambers with no chemoattractant). Graphs represent the means ± SEMs from at least three independent experiments, with each experiment performed in triplicate. ∗denotes p < 0.05.