Figure 10.

ATP6V1A knockdown has no effect on the production of rabies VLPs. HEK293T cells were transfected with ATP6V1A siRNA or scrambled siRNA for 48 h, and then transfected with plasmids of pcM and pcG at molar ratio of 6:1. At 48 h post transfection, cell culture supernatants were harvested and ultracentrifuged. Purified rabies VLPs were detected by Western blotting with murine serum against RABV M or G protein in denaturing conditions (A) or non-denaturing and non-reducing conditions (B). Purified rabies VLPs were incubated with mouse anti-G serum and gold-labeled goat anti-mouse IgG antibody, then stained with 2% phosphotungstic acid and observed by transmission electron microscopy (C). Cells, lysate of cells; Mock, cells as negative control without transfection and infection; super, purified supernatants of culture medium of cells.