Figure 7.

Identification of the domains of ATP6V1A and the M protein involved in their interaction. The fragment of amino acids 160 to 309 of ATP6V1A interacts with M protein. Schematic of the ATP6V1A truncates tagged with Myc and locations of the amino acid residues are noted (A). HEK293T cells were cotransfected with pM-NF and truncated constructs of ATP6V1A (i.e., pA1-159, pA160–309, pA310–464, or pA465–617), and the cell lysates were collected for coimmunoprecipitation assays using beads conjugated with anti-FLAG antibody (B–E) for FLAG-tagged M or Myc antibody for Myc-tagged ATP6V1A or its truncates (F and G). The N-terminal domain of the M protein interacts with ATP6V1A. Schematic of the M protein and its truncates fused to eGFP tagged with Myc, and the locations of the amino acids are noted (H). HEK293T cells were cotransfected with pA160-309-HA and the truncated M constructs pM1-76 (I) and pM77-202 (J); cell lysates were collected for coimmunoprecipitation assays using beads conjugated with Myc antibody.