Table 1.
Primary and amplification antibodies used for IHC
| Target | Antigen antibody/clone (supplier) | Antigen retrieval | Dilution | Secondary and amplification |
|---|---|---|---|---|
| Neurons | NeuN, Mouse monoclonal A60 (Millipore) |
Citrate, pH 6.0 HIER |
1:3000 | RbαM and GtαRb-HRP |
| Amyloid beta | 1–16 and 17–24 beta amyloid, Mouse monoclonal 6E10, and 4G8 (Biolegend) |
80% Formic acid | 1:1000 | RbαM and GtαRb-HRP |
| Microglia | IBA-1, Rabbit polyclonal (Wako) |
Citrate, pH 6.0 HIER |
1:2000 | DkαRb-bio and SA-HRP |
| Phospho-Tau | Phospho-Tau (Ser202, Thr205), mouse monoclonal AT8 (ThermoFisher) | Citrate, pH 6.0 HIER + 10-min PK |
1:1000 | RbαM and GtαRb-HRP |
| Astrocytes | GFAP, Rabbit polyclonal (Thermo Scientific) |
Citrate, pH 6.0 HIER |
1:200 | DkαRb-bio and SA-HRP |
GFAP, glial fibrillary acidic protein; IHC, immunohistochemistry; HIER, heat-induced antigen retrieval; PK, proteinase K; M, mouse; Rb, rabbit; Gt, goat; Dk, donkey; bio, biotin; SA, streptavidin; HRP, horseradish peroxidase.